Fluorimetric Estimation of Serum Creatine Kinase
نویسندگان
چکیده
منابع مشابه
Fluorimetric determination of creatine.
STRONGLY ALKALINE SOLUTIONS ninhydrin (1,2,3 indantrione hy) combines with guanidine, monosubstituted guanidines, and disubstituted guanidines to form highly fluorescent addition ucts (1). This reaction has been used as the basis for a simple, itive fluorimetric method for the determination of creatine in m, blood, and urine. he most widely used methods for determining creatine are based n the ...
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A 38-year-old white male taxi driver was referred to a lipid clinic by his general practitioner for management of combined hyperlipidemia. He was a smoker with a 20 packyear history. His father had died of myocardial infarction at age 55 years. Examination of this patient revealed a blood pressure of 136/99 mmHg and a body mass index of 29 kg/m, with no clinical signs of hyperlipidemia. Cholest...
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The purpose of this investigation was to develop a simple colorimetric method for creatine kinase (CK). The new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with different glyoxal compounds. Hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were...
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I studied activation of serum creatine kinase (CK) by sulfhydryl reagents, and the optimum assay conditions.Dithiothreitol and -mercaptoethanol were the best activators. Dithioerythritol, L-cys. teine,glutathione,and mercaptoacetatewere less effective. Over a dithiothreitol concentration range from 0.02 to 2 mmol/liter, enzyme activity increased with increasing dithiothreitol. Activation was ma...
متن کاملPairs of Creatine Kinase Serum Activity
Background: For patients with an increased risk of developing myopathy with statin treatment, guidelines recommend to measure CK before and 6-12 weeks after initiating statin treatment. Further, in clinical trials of statins, the number of patients experiencing elevated creatine kinase (CK) serum activity is commonly reported as a safety parameter. Usually exceeding the upper limit of normal (U...
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ژورنال
عنوان ژورنال: Proceedings of the Association of Clinical Biochemists
سال: 1967
ISSN: 0369-8564
DOI: 10.1177/036985646700400704